Bright Field Polarized transmitted LED illumination0 pages

 
Optilia Instruments AB

High Quality, Flexible Visual Inspection Systems 

Bright Field Illumination
In Bright Field illumination, light is transmitted (i.e., illuminated from below and observed from
above) and contrast in the image is caused by absorbance of some of the transmitted light in
dense areas of the specimen. The name "Bright field" is derived from the fact that the specimen
is dark (or colourful) and contrasted by the surrounding bright viewing field. Bright Field
illumination can be equipped with crossed polarizing filters (Polarizer/Analyzer). This illumination
technique can reveal details not visible using white light in some cases.
Bright field microscopy is well suited for following applications:
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Unstained or stained biological samples, such as water-borne single-celled organisms.
Precision semitransparent plastic parts.
Fibers, hairs, yeast and protozoa.
Minerals and crystals, thin polymers and some ceramics.
Forensics.
Examining external details, such as outlines, edges, grain
boundaries and surface defects. 
 
 

HD camera

Analyzer (if polarized illumination)

Image forming rays (blue)
Illumination beam (orange)

Working
Distance

Unstained Object
Object plane
Illumination beam
Background Illuminator
with or without Polarizer

RevA, Actober 2012